Insulin-secreting cells derived from embryonic stem cells normalize glycemia in streptozotocin-induced diabetic mice.

• DOI: 10.2337/diabetes.49.2.157
• Publication Date: 2007-03-06T19:04:22Z
• Authors: B Soria; E Roche; G Berná; T León-Quinto; J A Reig; F Martín
• Keywords: clone (Java method)

Embryonic stem (ES) cells display the ability to differentiate in vitro into a variety of cell lineages. Using cell-trapping system, we have obtained an insulin-secreting clone from undifferentiated ES cells. The construction used allows expression neomycin selection system under control regulatory regions human insulin gene. chimeric gene also contained hygromycin resistance (pGK-hygro) select transfected A resulting (IB/3x-99) containing 16.5 ng/microg protein total displays regulated hormone secretion presence various secretagogues. Clusters this were implanted (1 x 10(6) cells) spleen streptozotocin-induced diabetic animals. Transplanted animals correct hyperglycemia within 1 week and restore body weight 4 weeks. Whereas intraperitoneal glucose tolerance test showed slower recovery transplanted versus mice, blood normalization after challenge meal was similar. This approach opens new possibilities for tissue transplantation treatment type 2 diabetes offers alternative therapy.