Effects of Insulin Treatment in Type 2 Diabetic Patients on Intracellular Lipid Content in Liver and Skeletal Muscle

• DOI: 10.2337/diabetes.51.10.3025
• Publication Date: 2007-03-06T19:04:22Z
• Authors: Christian Anderwald; Elisabeth Bernroider; Martin Krs̆s̆ák; Harald Stingl; Attila Brehm; Martin G. Bischof; Peter Nowotny; Michael Roden; Werner Waldhäusl
• Keywords: Blood Glucose; Male; 0301 basic medicine; Magnetic Resonance Spectroscopy; Fatty Acids, Nonesterified; Middle Aged; 3. Good health; 03 medical and health sciences; Diabetes Mellitus, Type 2; Liver; Humans; Hypoglycemic Agents; Insulin; Female; Insulin Resistance; Muscle, Skeletal; Aged

Insulin resistance is frequently associated with increased lipid content in muscle and liver. excess stimulates tissue accumulation. To examine the effects of insulin improved glycemia on sensitivity intracellular lipids, we performed stepped (1, 2, 4 mU · min−1 kg−1) hyperinsulinemic-euglycemic clamps eight type 2 diabetic six nondiabetic control subjects at baseline after 12 67 h insulin-mediated near-normoglycemia (118 ± 7 mg/dl). Intrahepatocellular lipids (IHCLs) intramyocellular (IMCLs) soleus (IMCL-S) tibialis anterior (IMCL-TA) were measured 1H nuclear magnetic resonance spectroscopy. At baseline, had an approximate twofold higher (P < 0.02) lower IHCLs than patients (5.8 1.2 vs. 18.3 4.2%, P 0.03), whom IMCL-TA negatively correlated (r = −0.969, 0.001). After a 67-h infusion patients, IMCL-S 0.05) by ∼36 ∼18%, respectively, positively (IMCL-S: r 0.982, 0.0005; IHCL: 0.865, whereas fasting glucose production, d-[6,6-2H2]glucose, decreased ∼10% 0.04). In conclusion, these results indicate that IMCLs relate to for ∼3 days reduces production but accumulation liver without affecting sensitivity.