Insufficient Glycemic Control Increases Nuclear Factor-κB Binding Activity in Peripheral Blood Mononuclear Cells Isolated From Patients With Type 1 Diabetes

• DOI: 10.2337/diacare.21.8.1310
• Publication Date: 2007-03-05T22:51:27Z
• Authors: Marion A Hofmann; Stephan Schiekofer; Michael Kanitz; Martina S Klevesath; Martina Joswig; Vong Lee; Michael Morcos; Hans Tritschler; Reinhard Ziegler; Peter Wahl; Angelika Bierhaus; Peter P Nawroth
• Keywords: Adult; Blood Glucose; Glycated Hemoglobin; 0301 basic medicine; Thioctic Acid; NF-kappa B; Nuclear Proteins; In Vitro Techniques; Antioxidants; 3. Good health; 03 medical and health sciences; Cross-Sectional Studies; Diabetes Mellitus, Type 1; Diabetic Neuropathies; Leukocytes, Mononuclear; Humans; Regression Analysis; Lipid Peroxidation; Biomarkers

OBJECTIVE The redox-sensitive transcription factor nuclear factor-kB (NF-kB) is believed to contribute late diabetic complications. It unknown whether NF-kB influenced by glycemic control. RESEARCH DESIGN AND METHODS To determine activated in patients with insufficient control (HbA1c > 10%), we developed a tissue culture-independent electrophoretic mobility shift assay (EMSA)-based semiquantitative detection system that allowed us activation ex vivo-isolated peripheral blood mononuclear cells (PBMCs). We included 43 type 1 diabetes this cross-sectional study. 10 of those received the antioxidant thioctic acid (600 mg/day p.o.) for 2 weeks. RESULTS Monocytes HbA1c levels 10% demonstrated significantly higher binding activity an EMSA and stronger staining immunohistochemistry than monocytes 6–8%. increase correlated plasmatic markers lipid peroxidation. Treatment decreased activity. CONCLUSIONS Hyperglycemia induces PBMCs diabetes. at least partially dependent on oxidative stress, since lowered extent