<b>Effect of Marked Weight Loss on Adipose Tissue Biology in People with Obesity and Type 2 Diabetes</b>

• DOI: 10.2337/figshare.28641947.v1
• Publication Date: 2025-04-10T15:51:02Z
• Authors: Dmitri Samovski; Gordon I. Smith; Hector Palacios; Terri Pietka; Anja Fuchs; Gary J. Patti; Allah Nawaz; C. Ronald Kahn; Samuel Klein

&lt;p dir="ltr"&gt;&lt;b&gt;Objective&lt;/b&gt;: Weight loss improves insulin sensitivity in people with obesity and type 2 diabetes. However, the mechanisms responsible for this effect are unclear. We hypothesized that alterations adipose tissue biology tissue-related factors plasma involved mediating systemic metabolic benefits of weight loss.&lt;/p&gt;&lt;p dir="ltr"&gt;&lt;b&gt;Research Design Methods&lt;/b&gt;: evaluated blood samples obtained from ten adults diabetes before after marked (16%-20%) &gt;50% increase whole-body sensitivity, assessed by using hyperinsulinemic-euglycemic clamp procedure.&lt;/p&gt;&lt;p dir="ltr"&gt;&lt;b&gt;Results: &lt;/b&gt;Weight loss: &lt;a href="" target="_blank"&gt;i) &lt;/a&gt;&lt;a target="_blank"&gt;decreased &lt;/a&gt;expression genes related to extracellular matrix remodeling; ii) decreased expression SERPINE 1 which encodes plasminogen activator inhibitor-1 (PAI-1); iii) did not decrease immune cell content or inflammation; iv) ceramide content; v) target="_blank"&gt;PAI-1 &lt;/a&gt;and leptin concentrations increased high-molecular (HMW) adiponectin; vi) small vesicle (sEV) concentration sEV microRNAs proposed inhibit action, completely reversed inhibitory sEVs on signaling myotubes.&lt;/p&gt;&lt;p dir="ltr"&gt;&lt;b&gt;Conclusions: &lt;/b&gt;These findings suggest increases modifying concomitant circulating PAI-1, leptin, HMW adiponectin microRNAs.&lt;/p&gt;