MANAGEMENT FOR GAPOKTANHUT LESTARI SEJAHTERA: INITIAL OPTIMIZATION ON ROBUSTA COFFEE DNA AMPLIFICATION FOR MOLECULAR SPECIES CONFIRMATION

• DOI: 10.24233/biov.10.1.2024.429
• Publication Date: 2024-06-08T01:57:52Z
• Authors: Elly Lestari Rust...; Priyambodo; Dian Neli Pratiwi; Eko Agus Srihanto; Muhammad Febriansyah; Andri Wijaya Kusuma; Enny Saswiyanti; Alvin Wiwiet Susanto
• Keywords: Coffea canephora

Gapoktanhut Lestari Sejahtera, a community engaged in coffee plantation practices within the forests of Forest Protection Register 31 Pematang Arahan, Tanggamus, operates under forest management unit, KPH Kotaagung Utara, Lampung. In line with its commitment to biodiversity governance and preservation conservation biodiversity, species confirmation, particularly for robusta coffee, is deemed necessary. A leaf-based molecular confirmation was proposed, method yet be conducted Therefore, prior analysis, preliminary study focusing on temperature optimization DNA extraction undertaken. This DIPA BLU Unila Basic Research Grant 2023, collaboration Biotechnology Laboratory, Lampung Disease Investigation Center. Robusta leaves were sampled from six farmer groups, KTH Bumi Mulyo, Sido Makmur 1, 2, 3, Mandiri Jaya, Murah Rejeki Semaka, Tanggamus. The process adhered Genomic Mini Kit (Plant) protocol (ISO 9001: 2008 QMS). amplification performed using Polymerase Chain Reaction (PCR) method, utilizing Coffea N-methyltransferases gene marker primer. analysis leaf samples Sejahtera demonstrated good presence an 55°-60°C. To advance subsequent process, required yield improved results.