Considering the clarithromycin resistance prevalence among Helicobacter pylori strains isolated from patients with Gastric complication

• DOI: 10.25082/ccr.2024.01.005
• Publication Date: 2025-03-17T04:26:46Z
• Authors: Alireza Ahmadzadeh; Zhaleh Mohsenifar; Behzad Hatami; Ali Pirsalehi; Mostafa Rezaei-Ta...
• Keywords: Helicobacter Infections

Objective: Helicobacter pylori is a major cause of gastric cancer. This study aimed to determine the frequency clarithromycin resistance and its association with point mutations in 23S rRNA gene. Methods: was conducted on 100 patients disorders who were referred Valiasr Hospital 2022. Two biopsy samples obtained from each patient for pathological microbiological examinations. Antimicrobial susceptibility testing performed using agar dilution method, polymerase chain reaction (PCR) used molecular analysis.Results: Among patients, 53% (53/100) diagnosed as H. pylori-positive. Pathological findings indicated that 54.7% (29/53) pylori-positive had chronic gastritis, 37.7% (20/53) severe active 7.5% (4/53) intestinal metaplasia. Clarithromycin detected 13.2% (7/53) patients. The MIC50 MIC90 values determined be 0.125 mg/L 2 mg/L, respectively. PCR results revealed A2142G mutation gene present all clarithromycin-resistant strains. Conclusions: Our indicate presence may associated These underscore importance routine screening genotypes guide effective treatment strategies.