Alterations in the liver of intrauterine growth retarded piglets may predispose to development of insulin resistance and obesity in later life.

• DOI: 10.26402/jpp.2018.2.06
• Publication Date: 2018-04-01
• Authors: Karolina Ferenc; Piotr Pietrzak; Małgorzata Wierzb...; Piotr Matyba; Elżbieta Grzesiuk; Zdzisław Gajewski; R. Zabielski
• Keywords: Male; Proteomics; Fetal Growth Retardation; Kupffer Cells; Swine; Organ Size; Receptor, Insulin; Animals, Newborn; Liver; Animals; Cytokines; Uncoupling Protein 3; Female; Obesity; Insulin Resistance; Receptors, Adiponectin

Intrauterine growth retardation (IUGR) leads to increased predisposition metabolic syndrome in adult life but the mechanisms remain obscure. Considering a significant number of functional similarities, IUGR piglets appear be good model study development this humans. The aim present was investigate ultrastructure and proteomic profile liver pig neonates discover early markers obesity insulin resistance. In our intestine tissue samples were investigated 7 day old normal body weight (NBW) littermate using histometry, mass spectrometry, in-tissue cytometry analysis confocal microscopy. Compared NBW, characterized by significantly enhanced ratio Kupffer cells hepatocytes receptor abundance as well higher percentages expressing receptors for adipokines (resistin adiponectin), expression TNF-α (as marker inflammation), uncoupling protein 3 (UCP3). Moreover, NBW differed profile, including metabolism (proteasomes, cathepsin D, phermitin, phosphoglucomutase), carbohydrate (hexokinase 1, phosphoglucokinase, galactokinase, aldolase B, glucose-6-phosphate isomerase), oxidative stress chromatin organization DNA uptake (histones, lamin a/c). Reduction hepatocyte numbers concomitant with modifications key hormones enzymes may predispose resistance life.