HIV-1 integration site preferences in pluripotent cells

• DOI: 10.2741/e260
• Publication Date: 2010-11-28T18:56:35Z
• Authors: Rene Daniel
• Keywords: Pluripotent Stem Cells; 0301 basic medicine; Genomic Library; Virus Integration; Genetic Vectors; Genetic Therapy; Telomere; 03 medical and health sciences; Cell Line, Tumor; HIV-1; Humans; Cloning, Molecular; DNA Primers

HIV-1-based vectors are widely used in gene therapy. In somatic cells, these mainly integrate within genes. However, no distinct integration site preferences have been observed with regard to large chromosomal regions. The recent emergence of induced pluripotent stem (iPS) similar embryonic (ES) has raised questions about where occurs cells. this work we investigated the a pluripotent, ES-like cell line. We show that approximately 30% integrations occur vicinity telomeres. analyzed sites various as reported by us and other groups, pattern is unique conclude cells may contain cellular cofactors participate targeting not present