PTCH1, a receptor of Hedgehog signaling pathway, is correlated with metastatic potential of colorectal cancer

• DOI: 10.3109/03009731003668316
• Publication Date: 2010-03-15T08:45:49Z
• Authors: Sihong You; Jiannong Zhou; Senqing Chen; Ping Zhou; Jinghuan Lv; Xiao Han; Yujie Sun
• Keywords: PTCH1

Background. Approximately 90% of colorectal cancer (CRC) deaths arise from the metastatic dissemination primary tumors. It is difficult to predict metastasis cancer, especially for patients with same pathological subtype and differentiation. Aims. To identify biomarkers predicting CRC metastasis. Patients methods. We collected 19 tumors identical subtype, differentiation, comparable Dukes' stages matched age gender but completely different prognosis. were divided into one high-risk low-risk group The expression levels SHH, PTCH1, sFRP1, which are components Hedgehog signaling pathway, determined by real-time reverse transcription polymerase chain reaction (RT-PCR). investigate further correlation between level PTCH1 potential cells, we compared mRNA protein gene in LoVo cells high HT-29, SW480, SW620 low RT-PCR flow cytometry. Results. found that tumor tissues expressed lower than did those group. Similarly, inversely correlated cell lines. Expression SHH sFRP1 genes not differ two groups. Conclusion. Our data suggest might be a biomarker could discriminate risk.