The Indoleamine 2,3-dioxygenase Inhibitor 1-methyl-tryptophan Suppresses Mitochondrial Function, Induces Aerobic Glycolysis and Decreases Interleukin-10 Production in Human Lymphocytes

• DOI: 10.3109/08820139.2012.682244
• Publication Date: 2012-05-17T19:55:16Z
• Authors: Theodoros Elefthe...; Georgios Pissas; Aggeliki Karioti; Georgia Antoniadi; Vassilios Liakopo...; Konstantina Dafop...; Spyros Pournaras; Georgios Koukoulis; Ioannis Stefanidis
• Keywords: Indoleamine 2,3-dioxygenase; Anaerobic glycolysis

Indoleamine 2,3-dioxygenase (IDO) suppresses adaptive immunity. It is known that IDO induces T-cell differentiation to regulatory T-cells (Treg) through tryptophan depletion and/or kynurenine pathway products. CD4+ effector require distinct metabolic programs in order support their function as compared Treg cells. Furthermore, glucose metabolism also affect B-cell survival and function. The effect of on lymphocytes was evaluated by using its inhibitor 1-methyl-DL-tryptophan (1-MT).Ten healthy volunteers vaccinated against tetanus. Peripheral blood mononuclear cells (PBMC) were cultured with or without tetanus toxoid 1-MT. Cell proliferation assessed optical microscopy, uptake measuring concentration the supernatant, aerobic glycolysis assessing lactate mitochondrial XTT assay, finally production Tregs' signature cytokine IL-10 means ELISA.Primarily, decreases stimulated lymphocytes. Secondly, increases In addition, Finally, immunosuppressive lymphocytes.Considering cell plays a significant role lymphocyte function, may exert immunomodulatory interfering metabolism.