Paricalcitol may improve oxidative DNA damage on experimental amikacin-induced nephrotoxicity model

• DOI: 10.3109/0886022x.2016.1158071
• Publication Date: 2016-03-17T06:01:31Z
• Authors: Gulay Bulut; Yildiray Basbugan; Elif Ari; Remzi Erten; Havva Bektas; Hamit Hakan Alp; Irfan Bayram
• Keywords: Nephrotoxicity; Amikacin; Paricalcitol

This study aimed to investigate the possible protective effect of paricalcitol on experimental amikacin-induced nephrotoxicity model in rats. Wistar albino rats (n = 32) were allocated into four equal groups eight each, control (Group C), P), A), and paricalcitol-treated A + P) groups. Paricalcitol was given intra-peritoneally at a dose 0.4 μg/kg/d for 5 consecutive days prior induction nephrotoxicity. Intra-peritoneal amikacin (1.2 g/kg) used induce day 4. Renal function parameters, oxidative stress biomarkers, DNA damage (8-hydroxy-2'-deoxyguanosine/deoxyguanosine ratio), kidney histology, vascular endothelial growth factor (VEGF) immunoexpression determined. Group P had lower mean fractional sodium excretion (p < 0.001) as well higher creatinine clearance 0.026) than group A). tissue malondialdehyde levels 0.035) serum 8-hydroxy-2'-deoxyguanosine/deoxyguanosine ratio (8-OHdG/dG ratio) significantly lower; superoxide dismutase 0.024) glutathione peroxidase 0.007) activities renal A. The scores tubular necrosis 0.024), proteinaceous casts 0.038), medullary congestion 0.035), VEGF 0.018) also when compared with demonstrates prevention an model. Furthermore, it is first demonstrate that improves acute injury