Osteogenically-induced human dermal fibroblasts as a tool to regenerate bone

• DOI: 10.3109/2000656x.2012.731411
• Publication Date: 2013-01-18T09:21:38Z
• Authors: Pehr Sommar; Johan P. E. Junker; Eivind Strandenes; Charlotte Ness; Thomas Hansson; Hans Johnson; Gunnar Kratz
• Keywords: Microcarrier; Osteonectin

Engineering of bone tissue could help to overcome the need for extensive reconstruction and associated donor site morbidity, it has been proposed that osteogenic biomaterials, which are scaffolds contain osteocompetent cells, be used fill large defects. This study investigated potential osteogenically-induced human dermal fibroblasts cultured on gelatin microcarriers combined with platelet-rich plasma in a model femoral defect athymic rats. Defects were transplanted one following six combinations: 1 = sodium chloride, 2 plasma, 3 + 4 5 osteoblasts 6 osteogenically induced plasma. The defects assessed weeks postoperatively computed tomography (CT), routine histological staining, fluorescence situ hybridisation, polyclonal antibodies directed towards osteocalcin osteonectin. Radiographs all groups taken showed unhealed Femoral (group 6) contained dense clusters cells quantities extracellular matrix. These exclusive this group stained strongly Fluorescence hybridisation viable had seeded confirmed survival implanted cells. In conclusion, survived new niche, bone-like structures apparent