Phylogenies and Chromosome Evolution ofPhacelia(Boraginaceae: Hydrophylloideae) Inferred from Nuclear Ribosomal and Chloroplast Sequence Data

• DOI: 10.3120/0024-9637-61.1.16
• Publication Date: 2014-03-29T17:21:48Z
• Authors: Genevieve K. Walden; Laura M. Garrison; Greg S. Spicer; Frank W. Cipriano; Robert Patterson
• Keywords: NdhF; Maximum parsimony; Coalescent theory

This project sampled throughout Phacelia using the internal transcribed spacer region (ITS-1, ITS-2, and 5.8S gene) of nuclear ribosomal DNA (nrITS) chloroplast gene (ndhF) to infer phylogenies for plastid partitions. Nuclear partitions were incongruent in our analyses. Phylogenetic analyses (maximum parsimony, maximum likelihood, Bayesian inference) recovered tree topologies similar previous molecular studies. We corroborate incongruence between placement some problematic groups (e.g., Draperia, Romanzoffia "core" subg. Pulchellae, sect. Baretiana). Combined resulted better resolution than separate analyses, a topology that favored topologies. was sister monophyletic combined Our results support combining analysis seek nodes. Maximum likelihood used ancestral chromosome numbers identify gains, losses, polyploid doubling, whole genome duplication events from published counts genus. The predicted base number genus x = 9, 11, or 12.