Mortality of Pond-cultured <i>Litopenaeus vannamei</i> Associated with Acute Hepatopancreatic Necrosis Disease (AHPND) and White Spot Syndrome Virus (WSSV) Infection in the Philippines

• DOI: 10.3147/jsfp.52.38
• Publication Date: 2017-04-03T18:14:36Z
• Authors: Leobert D. de la ...; Nikko Alvin R. Ca...; Edgar C. Amar; Demy D. Catedral; Roselyn C. Usero; Joseph P. Faisan,...; Joey I. Arboleda; Wilberto D. Monot...; Adelaida T. Calpe; Dalisay D. G. Fer...; Cynthia P. Saloma
• Keywords: Litopenaeus; White spot syndrome; Amplicon; Penaeus

Mortalities of up to 60% were observed in pond-cultured Litopenaeus vannamei Bohol, Philippines. Histopathological examination revealed typical acute hepatopancreatic necrosis disease (AHPND) pathology. PCR test generated 1,269 bp and 230 amplicons confirmative for the toxin-producing AHPND strain Vibrio parahaemolyticus among shrimp sampled from eight ponds. The same samples subjected analyses presence other viruses, namely WSSV, IHHNV, IMNV, TSV. negative viruses except which was detected after one-step six out These results suggested that infected dually with V. WSSV.