Analysis and Validation of the Prognosis ability of the M7GRelated miRNAs in Lung Adenocarcinoma

• DOI: 10.31557/apjcp.2023.24.4.1275
• Publication Date: 2023-04-28T20:03:37Z
• Authors: Xiaowen Qiu; Yongting Chen; Xingzhuang Zhu; Zheng Gong; Fengyuan Yu; Pengfei Zhang; Yipeng Song; Hongwei Li
• Keywords: Nomogram; KEGG; Univariate

Background: N7-methylguanosine (m7G) modification plays a crucial role in the development and progression of lung cancers. MicroRNAs (miRNAs) are closely involved programmed cell death mechanism tumor growth. The m7G-associated miRNAs genes adenocarcinoma (LUAD), their prognosis prediction ability LUAD, however, had not been investigated. Methods: RNA transcriptomes, clinical indices, immune scores LUAD patients were searched downloaded from Cancer Genome Atlas (TCGA) ESTIMATE database. targeting METTL1 WDR4 extracted TargetScan Differentially expressed m7G-related identified power was systematically Results: Among 40 differentially five (hsa-miR-31-5p, hsa-miR-5571-3p, hsa-miR-4697-3p, hsa-miR-6858-5p, hsa-miR-873-3p) demonstrate significant predictive value for prognosis. risk score constructed by these an independent prognostic factor (univariate Cox regression results: hazard ratio (HR) = 1.6619, 95% confidential interval (CI) 1.2103-2.2819, p 0.0017; multivariate HR 1.6004, CI 1.1633-2.2017, 0.0039). survival curves showed that with high-risk poor Calibration indicated good predictability nomogram combining miRNA indices age, sex, chemotherapy, radiotherapy, stage, score. GO KEGG analysis overlapping associated neuropeptide signaling pathway. Besides, infiltration expression AMPD1 gene strongly cells immunology functions LUAD. Conclusion: This study DE demonstrated patients. signature based on demonstrates high accuracy predicting