Supplementation of parenteral nutrition with fish oil attenuates acute lung injury in a rat model

• DOI: 10.3164/jcbn.13-90
• Publication Date: 2014-01-24T23:10:51Z
• Authors: Keisuke Kohama; Atsunori Nakao; Mariko Terashima; Michiko Aoyama-Is...; Takayuki Shimizu; Daisuke Harada; Mitsuo Nakayama; Hayato Yamashita; Mayu Fujiwara; Joji Kotani
• Keywords: Leukotriene B4

Fish oil rich in n-3 polyunsaturated fatty acids has diverse immunomodulatory properties and attenuates acute lung injury when administered enternal nutrition. However, enteral nutrition is not always feasible. Therefore, we investigated the ability of parenteral supplemented with fish to ameliorate injury. Rats were infused solutions (without lipids, soybean oil, or oil) for three days. Lipopolysaccharide (15 mg/kg) was then intratracheally induce injury, characterized by impaired function, polymorphonuclear leukocyte recruitment, parenchymal tissue damage, upregulation mRNAs inflammatory mediators. Administration prior insult improved gas exchange inhibited neutrophil recruitment Parenteral also prolonged survival. To investigate underlying mechanisms, leukotriene B4 B5 secretion measured neutrophils from peritoneal cavity. The rats treated oil-rich released significantly more B5, an anti-inflammatory eicosanoid, than isolated given standard reduced lipopolysaccharide-induced part promoting synthesis eicosanoids.