Effects of a multispecies direct-fed microbial on gastrointestinal permeability during feed restriction in a growing heifer model

• DOI: 10.3168/jdsc.2023-0402
• Publication Date: 2023-11-17T21:26:07Z
• Authors: B.M. Goetz; M.A. Abeyta; S. Rodriguez-Jimenez; J. Opgenorth; J.L. McGill; K.A. Bryan; L.H. Baumgard
• Keywords: Haptoglobin

Objectives were to evaluate the effects of a 4-strain direct fed microbial (DFM) on gastrointestinal tract (GIT) permeability and inflammation during feed restriction (FR) in heifers. Holstein heifers (n = 32; mean ± SD; 295 25 kg BW; 287 17 d age) utilized an experiment conducted 2 replicates (16/replicate). Heifers randomly assigned 1 top-dressed dietary treatments: 1) control (CON; 10 g/d dried lactose; n 16) or 2) DFM containing commercial blend Lactobacillus animalis, Propionibacterium freudenreichii, Bacillus licheniformis, subtilis at 11.8 × 109 cfu/day (PRO; DFM; 16). The trial consisted experimental periods (P): P1 (14 d) served as baseline for P2 (5 d), when all restricted 40% their DMI. On 12 5, GIT was evaluated using oral chromium (Cr)-EDTA. By design, FR decreased DMI (60%) BW (~18 kg) Regardless treatment, FR, had circulating glucose, BHB, insulin, L-lactate (4, 14, 45, 19%, respectively), but increased nonesterified fatty acids, serum amyloid A haptoglobin (3.0-, 1.7-, 5.0-fold, respectively). Circulating white blood cells, neutrophils, lymphocytes, basophils 7, 6%, respectively) whereas eosinophils (41%) irrespective treatment. IFN-γ inducible protein-10 (23%) compared with regardless Plasma Cr area under curve 5 (10 14%, relative P1, this unaltered by In summary, compromised barrier function stimulated inflammatory response, did not appear be ameliorated PRO.