Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins

• DOI: 10.3201/eid2501.180605
• Publication Date: 2018-11-02T10:57:51Z
• Authors: Alexandra Rockstroh; Luisa Barzon; Widuranga Kumbukg...; Hoang Xuan Su; Erley Lizarazo; Maria Fernanda Vi...; Adriana Tami; Alice M.M. Ornelas; Renato Santana Ag...; Daniel Cadar; Jonas Schmidt-Cha...; Sebastian Ulbert
• Keywords: Immunoglobulin M

We developed an IgM-based ELISA that identifies the dengue virus serotype of recent infections. Dominant serotypes were detectable in 91.1% samples from travelers and 86.5% residents endemic regions; 97.1% corresponded to identified by PCR. This enables more accurate reporting epidemiologic findings.