MicroRNA-761 inhibits Angiotensin II-induced vascular smooth muscle cell proliferation and migration by targeting mammalian target of rapamycin

• DOI: 10.3233/ch-151981
• Publication Date: 2016-07-01T14:51:10Z
• Authors: Jung Rae Cho; Chang Yeon Lee; Jiyun Lee; Hyang-Hee Seo; Eunhyun Choi; Namsik Chung; Sung-Man Kim; Ki-Chul Hwang; Seahyoung Lee
• Keywords: 0301 basic medicine; Angiotensin II; TOR Serine-Threonine Kinases; Transfection; Muscle, Smooth, Vascular; Rats; 3. Good health; Rats, Sprague-Dawley; MicroRNAs; 03 medical and health sciences; Animals; Cell Proliferation; Signal Transduction

Aberrant vascular smooth muscle cell (VSMC) proliferation and migration are a major pathological phenomenon in disease characterized by intimal thickening. The important role of the mammalian target rapamycin (mTOR) signaling VSMC has been previously reported. Consequen tly, down-regulation mTOR pathway may be an effective way controlling excessive proliferation. Since microRNAs (miRNA) newly emerging regulators virtually all biological processes including cellular proliferation, miRNAs targeting utilized to suppress aberrant during pathologic conditions. Thus, present study, we screened mTOR, identified miR-761 as new miRNA. Luciferase assay using luciferase vector containing 3'UTR indicated that directly targets mRNA leading suppression protein expression. Our data also indicate expression decreases angiotensin II (AngII)-induced VSMCs, exogenous delivery effectively inhibit AngII-induced Additionally, results tests demonstrate suppresses VSMCs well. Taken together, study provided evidence can potent anti-proliferative agent for diseases such atherosclerosis restenosis, warrants further studies validate effectiveness vivo.