In vitro propagation of Xao tam phan (Paramignya trimera (Oliv.) Guill.)

• DOI: 10.32508/stdjns.v1it2.452
• Publication Date: 2018-09-18T06:49:26Z
• Authors: Hieu Trung Tran; Chung Van Huynh; Hue Thi Linh Bui; Ngan Thi My Luong; Anh Lan Bui; Le Van Bui
• Keywords: Callus

Paramignya trimera (Oliv.) Guill., a woody climber commonly known as "Xao tam phan", has been used in Vietnamese folk for the treatment of numerous cancers. Due to word mouth about anticancer properties this plant, its stems and roots have overexploited leading serious decline species Phu Yen, Khanh Hoa Ninh Thuan provinces. The aim study was establish an vitro propagation protocol conservation P. trimera. In research shoot clusters (5–8 shoots/cluster) were regenerated from axillary bud explants 1–3 year-old trees after 3 months cultures on WPM (woody plant medium) supplemented with STS BA 5–7 mg/L. (silver thiosulfate) prevent leaf abscission. These grew slowly reached cm heights 4 cultures. did not form any 2 culture rooting media IBA and/or NAA 1–5 However, there 51 % treated acclimatized produced new stem leaves growing greenhouse. 3, 5 mg/L showed best response callus induction Among types, milky white compact calli induced at cut surface became nodulated within weeks later.