Transcriptome Analysis and Genes Function Verification of Root Development of Paeonia suffruticosa under Sandy Loam Cultivation

• DOI: 10.32604/phyton.2022.023572
• Publication Date: 2022-08-29T07:36:41Z
• Authors: Yinglong Song; Wenqian Shang; Zheng Wang; Songlin He; Xinya Meng; Liyun Shi; Yuxiao Shen; Dan He; Xueyuan Lou; Yuke Sun
• Keywords: Paeonia suffruticosa; Primordium; KEGG

Relatively poor in vitro rooting has limited the large-scale commercial production of tree peony. In this study, on basis transcriptome sequencing, differentially expressed genes and associated metabolic pathways were identified peony roots at different stages root formation under sandy loam cultivation. A total 31.63 Gb raw data generated 120,188 unigenes (mean length 911.98 bp) annotated according to six databases (NR, NT, GO, KEGG, COG, Swiss-Prot). Analyses ungerminated primordium period, induced period detected 8,232, 6,907, 10,687 related 133, 132, 133 pathways, respectively. Two significantly (Unigene13430_All CL10096.Contig1_All) with auxin pathway. The full-length Unigene13430_All coding sequence (843 encoded 280 amino acids, whereas CL10096.Contig1_All (1,470 489 acids. as IAA gene family members respectively named PsIAA27 PsARF19. qRT-PCR analysis functional verification indicated that expressions PsARF19 seedlings, cuttings grafted seedlings significant different. promoted development, it might be a regulatory roots, while inhibited lateral involved controlling sensitivity during formation. results study may form future investigations mechanism mediating will an excellent resource for researchers interested characterizing rooting-related genes.