Bladder Regeneration Using a Polycaprolactone Scaffold with a Gradient Structure and Growth Factors in a Partially Cystectomized Rat Model

• DOI: 10.3346/jkms.2020.35.e374
• Publication Date: 2020-10-19T00:09:08Z
• Authors: Ho Yong Kim; So Young Chun; Eun Hye Lee; Bomi Kim; Yun-Sok Ha; Jae-Wook Chung; Jun Nyung Lee; Bum Soo Kim; Se Heang Oh; Tae Gyun Kwon
• Keywords: Polycaprolactone

Background Tissue engineering can be used for bladder augmentation. However, conventional scaffolds result in fibrosis and graft shrinkage. This study applied an alternative polycaprolactone (PCL)-based scaffold (diameter = 5 mm) with a noble gradient structure growth factors (GFs) (epidermal factor, vascular endothelial basic fibroblast factor) to enhance tissue regeneration rat model. Methods Partially excised urinary bladders of 5-week-old male Slc:SD rats were reconstructed the (scaffold group) or combined GFs (GF compared sham-operated (control untreated (partial cystectomy group). Evaluations volume, histology, immunohistochemistry (IHC), molecular markers performed at 4, 8, 12 weeks after operation. Results The volumes GF group recovered normal range, those showed more enhanced Histological evaluations revealed that organized urothelial lining, dense extracellular matrix, frequent angiogenesis, smooth muscle bundle than group. IHC α-smooth actin, pan-cytokeratin, α-bungarotoxin, CD8 high formation muscle, blood vessel, urothelium, neuromuscular junction low immunogenicity. Concordantly, real-time polymerase chain reaction experiments higher expression transcripts associated differentiation. In 6-month vivo safety analysis, histology. Conclusion PCL incorporating improved functionally histologically.