Identification of Carboxylesterase genes and their expression profiles in conferring resistance to commonly used pesticides in Zonocerus variegatus (linneaus, 1758)

• DOI: 10.33472/afjbs.3.4.2021.76-87
• Publication Date: 2021-10-18T10:41:33Z
• Authors: Folorunso T. R.; Awodiran M. O.
• Keywords: Carboxylesterase; Insecticide Resistance; Identification

Identifying insecticide resistance mechanisms is paramount for pest insect control, as the understandings that underpin control strategies must provide ways of detecting and managing resistance, thus study identified ability Carboxylesterase (CarEs) genes in conferring to commonly used pesticide by examining expression gene susceptible resistant strain Zonocerus variegatus, after Cypermethrin bioassay.Adult Z. variegatus was sampled from Savannah area Oyo State, Nigeria, samples were collected preserved "RNA Later" RNA extraction.The two groups later subjected quantitative Polymerase Chain Reaction (qPCR) using primers.CarEs expressions have both negative positive ct value when LmcesA20 primer used.However, no significant difference level observed between primers LmcesE9 (F = 0.84, p > 0.05) 0.499, 0.05).The concluded since CarEs same groups, may not function detoxifying variegatus.LmcesA20 showed a little variation strains having higher fold change than strain, these members need be further verified their specific role use pesticides.