Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous Administration

• DOI: 10.3348/kjr.2002.3.3.194
• Publication Date: 2009-07-17T04:18:33Z
• Authors: Bo Yoon Choi; Jin Wook Chung; Jae Hyung Park; Keon Ha Kim; Young Il Kim; Young Hwan Koh; Jong Won Kwon; Kyoung Ho Lee; Hyuk Jae Choi; Tae Woo Kim; Young Jin Kim; Hesson Chung; Ik Chan Kwon; Seo Young Jeong
• Keywords: Cationic polymerization; Systemic administration; Lipid emulsion

Gene Delivery to the Rat Liver Using Cationic Lipid Emulsion/DNA Complex: Comparison between Intra-arterial, Intraportal and Intravenous AdministrationObjective: To compare efficiency of intra-arterial, intraportal, intravenous administration cationic lipid emulsion/DNA complex, as used for gene transfer rat liver.Materials Methods: DNA-carrier complex in-vivo experiment was prepared by mixing DNA a emulsion.According route (intra-arterial, or intravenous), animals were assigned one three groups.The heart, lung, liver, spleen kidneys removed assayed total protein luciferase concentration. Results:The successfully transfected various organs via different routes employed.Luciferase activity in each organ untreated negligible.Liver values significantly higher groups which intra-arterial intraportal used.