Establishment of a Protocol for Determining Gastrointestinal Transit Time in Mice Using Barium and Radiopaque Markers

• DOI: 10.3348/kjr.2013.14.1.45
• Publication Date: 2013-01-07T09:56:16Z
• Authors: Bolormaa Myagmarj...; Myeong Ju Moon; Suk Hee Heo; Seo In Jeong; Jong-Seong Park; Jae Yeoul Jun; Yong Yeon Jeong; Heoung Keun Kang
• Keywords: Barium; Transit time; Barium sulfate; Gastrointestinal transit

The purpose of this study was to establish a minimally invasive and reproducible protocol for estimating the gastrointestinal (GI) transit time in mice using barium radiopaque markers.Twenty 5- 6-week-old Balb/C female weighing 19-21 g were used. animals divided into three groups: two groups that received loperamide control group. group (n = 10) administered physiological saline (1.5 mL/kg) orally. I II 5 mg/kg 10 orally, respectively. Thirty minutes after receiving or loperamide, 80 µL solution six iron balls (0.5 mm) via mouth upper esophagus by gavage, Afterwards, continuously monitored with fluoroscopic imaging order evaluate swallowing markers. Serial images obtained at 10-min intervals until all markers had been excreted from anal canal. For analysis, GI times subdivided intestinal (ITTs) colon (CTTs).The mean ITT significantly longer than (p < 0.05). (174.5 ± 32.3) (133.2 24.2 minute) CTT Also, no animal succumbed death experimental procedure.The our is determining mouse model.