Mechanical response of porous scaffolds for cartilage engineering

• DOI: 10.33549/physiolres.931297
• Publication Date: 2021-11-07T08:28:03Z
• Authors: J Jančář; A Slovíková; E Amler; P Krupa; H Kecová; L Planka; P Gal; A Nečas
• Keywords: 0301 basic medicine; Chitosan; Tissue Engineering; Biocompatible Materials; Mesenchymal Stem Cells; Collagen Type I; Elasticity; Prosthesis Failure; 03 medical and health sciences; Cartilage; Durapatite; Freeze Drying; Tensile Strength; Materials Testing; Animals; Humans; Nanoparticles; Cattle; Hyaluronic Acid; Porosity; Cells, Cultured; Cell Proliferation

Mechanical properties of scaffolds seeded with mesenchymal stem cells used for cartilage repair seem to be one the critical factors in possible joint resurfacing. In this paper, effect adding hyaluronic acid, hydroxyapatite nanoparticles or chitosan nanofibers into cross-linked collagen I on mechanical response lyophilized porous scaffold has been investigated dry state at 37 oC under tensile loading. Statistical significance results was evaluated using ANOVA analysis. The showed that addition acid significantly (p<<0.05) reduced elastic modulus and enhanced strength deformation failure modified test conditions. On other hand, nanofibers, respectively, increased ten-fold four-fold, respectively. Hydroxyapatite caused significant reduction ultimate break while loading substantially (p<<0.05). by nanofibers. translated resistance against load compared based neat similar porosity. It can concluded enhancing rigidity compact material rigid improve compressive loading, which provide more structural protection when construct is implanted a lesion. Moreover, seemed enhance cell growth tested vitro as well stability, extending its resorption than 10 weeks.