Silymarin Component 2,3-dehydrosilybin Attenuates Cardiomyocyte Damage Following Hypoxia/Reoxygenation by Limiting Oxidative Stress

Hypoxia Propidium iodide Cardioprotection Ischemic Preconditioning Silybum marianum
DOI: 10.33549/physiolres.932703 Publication Date: 2020-04-06T07:17:47Z
ABSTRACT
Ischemic postconditioning and remote conditioning are potentially useful tools for protecting ischemic myocardium. This study tested the hypothesis that 2,3-dehydrosilybin (DHS), a flavonolignan component of Silybum marianum, could attenuate cardiomyocyte damage following hypoxia/reoxygenation by decreasing generation reactive oxygen species (ROS). After 5-6 days cell culture in normoxic conditions rat neonatal cardiomyocytes were divided into four groups. Control group (9 h at conditions), (3 1 % O2, 94 N2 5 CO2 followed 10 min µmol·l-1 DHS 6 reoxygenation normoxia) hypoxia, three cycles hypoxia normoxia). Cell viability assessed propidium iodide staining was decreased after treatment consistent with increased levels lactatedehydrogenase (LDH) reoxygenation. LDH leakage significantly reduced when H/Re exposed to DHS. H2O2 production also ROS as evidenced fluorescence indicator. reduces reoxygenation-induced injury attenuation generation, protein carbonyls levels. In addition, we found both protocol associated restored ratio phosphorylated/total kinase C epsilon, relative group. conclusion, our data support protective role hypoxia/reperfusion indicate may act mimic.
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