Attenuating effect of melatonin on lipopolysaccharide-induced chicken small intestine inflammation

• DOI: 10.3382/ps/pey084
• Publication Date: 2018-03-27T02:33:28Z
• Authors: R.X. Li; J. Li; S.Y. Zhang; Y.L. Mi; C.Q. Zhang
• Keywords: Inflammation; Lipopolysaccharides; 0301 basic medicine; 2. Zero hunger; Apoptosis; Animal Feed; Epithelium; Diet; Immunoglobulin A; Avian Proteins; Toll-Like Receptor 4; 03 medical and health sciences; Animals, Newborn; Dietary Supplements; Intestine, Small; Animals; Goblet Cells; Intestinal Mucosa; Chickens; Poultry Diseases; Cell Proliferation; Melatonin

Enriched melatonin (MEL) has been found in the mammalian intestine and recently demonstrated to alleviate rodent colitis. In this study, effect of MEL on lipopolysaccharide (LPS)-induced intestinal inflammations was investigated new chicken hatchlings. The chicks were fed with a diet supplemented (12.5 mg/day) from D1 D10. Meanwhile, LPS or + groups injected (10 mg/kg BW, i.p.) at treatment for 6 h increased expression IL-6, IL-4, caspase-3 mRNAs TUNEL-positive cell populations, but decreased populations goblet PCNA+ cells, IgA production MUC2 mRNA duodenum. Compared group, pre-feeding alleviated duodenal inflammation TNF-α by 23.6% (P = 0.004), IL-6 69.4% 0.001), IL-4 4.1% 0.824) 45.8% < 0.001). Conversely, attenuated LPS-induced changes 161.6% 0.013) 172.1% 0.001) TLR4 also up-regulated down-regulated pre-feeding. conclusion, dietary could attenuate chick down-regulating inflammatory cytokines, promoting epithelial proliferation, improving immunological barrier inhibiting apoptosis via mediation TLR4.