Fabrication and Characterization of Collagen/PVA Dual-Layer Membranes for Periodontal Bone Regeneration

• DOI: 10.3389/fbioe.2021.630977
• Publication Date: 2021-06-09T08:30:17Z
• Authors: Tian Zhou; Siwei Chen; Xinxin Ding; Zhihuan Hu; Lian Cen; Xiaomeng Zhang
• Keywords: Barrier membrane

Guided tissue regeneration (GTR) is a promising treatment for periodontal defects, which generally uses membrane to build mechanical barrier from the gingival epithelium and hold space especially tooth-supporting bone. However, existing membranes possess insufficient properties limited bioactivity bone regenerate. Herein, fish collagen polyvinyl alcohol (Col/PVA) dual-layer were developed via combined freezing/thawing layer coating method. This had clear but contact boundary line between PVA layers, both hydrophilic. The dual an elongation at break of 193 ± 27% would undergo in vitro degradation duration more than 17 days. Further cell experiments showed that compared with layer, not only presented good cytocompatibility rat marrow-derived mesenchymal stem cells (BMSCs), also promoted osteogenic genes (RUNX2, ALP, OCN, COL1) protein (ALP) expression BMSCs. Hence, currently could be used as stable rate selectively favor repopulate defect. meet challenges encountered by GTR superior defect repair, demonstrating great potential clinical applications.