Revising Protein Corona Characterization and Combining ITC and Nano-DSC to Understand the Interaction of Proteins With Porous Nanoparticles

• DOI: 10.3389/fbioe.2021.650281
• Publication Date: 2021-10-12T10:16:13Z
• Authors: Alba Balmori; Romica Sandu; Daniela Gheorghe; Alina Botea-Petcu; Aurica Precupas; Speranta Tanasescu; David Sánchez-García; Salvador Borrós
• Keywords: Isothermal Titration Calorimetry; Nanomaterials; Protein Adsorption; Denaturation (fissile materials); Globular protein

The exposure of nanoparticles (NPs) to biological fluids leads the formation a protein coating that is known as corona (PC). Since PC influenced by physicochemical properties nanoparticles, understanding interplay factors participate in this process crucial for development nanomaterials cell-targeted delivery vehicles. In general, it accepted complex and dynamic process, which depends on composition medium NP mainly size, shape, superficial charge. Interestingly, although interaction between essentially phenomenon, influence roughness nanoparticle surface has been scarcely studied. work, porosity studied with aid nanodifferential scanning calorimetry (nano-DSC) isothermal titration (ITC) using mesoporous silica (MSNs) an model. proteins was analyzed ITC measurements, while stability denaturation monitored nano-DSC. Thanks complementarity these two techniques, more complete insight into pores accomplished.