Establishment of an Ex Vivo Inflammatory Osteoarthritis Model With Human Osteochondral Explants

• DOI: 10.3389/fbioe.2021.787020
• Publication Date: 2021-12-21T06:24:55Z
• Authors: Kaihu Li; Penghui Zhang; Yong Zhu; Mauro Alini; Sibylle Grad; Zhen Li
• Keywords: Aggrecan; Ex vivo; MMP3

Osteoarthritis (OA) is the most common degenerative joint disease without clear pathophysiological mechanism and effective drugs for treatment. Although various animal models exist, translation of outcome into clinics remains difficult due to species differences. In this study, an ex vivo inflammatory OA model was induced using different concentrations interleukin one beta (IL-1β) tumor necrosis factor α (TNF-α) on explants from human femoral head. groups, gene expression levels cartilage catabolism (matrix metalloproteinase 1 (MMP1), matrix 3 (MMP3)), inflammation (interleukin 6 (IL-6), 8 (IL-8)) markers were significantly upregulated, while anabolic genes (collagen 2 (COL2), aggrecan (ACAN), proteoglycan 4 (PRG4)) downregulated compared control group. The release cytokines (IL-6, IL-8) nitric oxide (NO) in conditioned medium also upregulated groups. Safranin O/Fast Green staining showed loss superficial zone after cytokine results indicated that degeneration successfully established osteochondral This can be used elucidate in-depth screen new