Macrophage-Derived Exosomes Promote Bone Mesenchymal Stem Cells Towards Osteoblastic Fate Through microRNA-21a-5p

• DOI: 10.3389/fbioe.2021.801432
• Publication Date: 2022-01-05T06:53:37Z
• Authors: Kun Liu; Xin Luo; Zhao-Yong Lv; Yu-Jue Zhang; Zhen Meng; Jun Li; Chun-Xiu Meng; Hui-Fen Qiang; Cai-Yao Hou; Lei Hou; Feng-Zhen Liu; Bin Zhang
• Keywords: Microvesicle; M2 Macrophage

The effective healing of a bone defect is dependent on the careful coordination inflammatory and bone-forming cells. In current work, pro-inflammatory M1 anti-inflammatory M2 macrophages were co-cultured with primary murine mesenchymal stem cells (BMSCs), in vitro, to establish cross-talk among polarized BMSCs, as well their effects osteogenesis. Meanwhile, influence osteogenesis BMSCs through paracrine forms such exosomes. We focused whether exosomes promote osteogenic differentiation. results indicated that macrophage all can BMSCs. Especially, macrophage-derived microRNA-21a-5p at early stage inflammation. This research helps develop an understanding intricate interactions macrophages, which help improve process additional regenerative processes by local sustained release