The Biosynthesis of D-1,2,4-Butanetriol From d-Arabinose With an Engineered Escherichia coli

• DOI: 10.3389/fbioe.2022.844517
• Publication Date: 2022-03-29T05:29:07Z
• Authors: Jing Wang; Qiaoyu Chen; Xin Wang; Kequan Chen; Pingkai Ouyang
• Keywords: Metabolic Engineering; Arabinose; Sulfolobus solfataricus; Industrial microbiology; Strain (injury)

D-1,2,4-Butanetriol (BT) has attracted much attention for its various applications in energetic materials and the pharmaceutical industry. Here, a synthetic pathway biosynthesis of BT from d-arabinose was constructed optimized Escherichia coli. First, E. coli Trans1-T1 selected synthesis BT. Considering different performance enzymes organisms when expressed coli, optimized. After screening two dehydrogenases (ARAs), d-arabinonate dehydratases (ADs), four 2-keto acid decarboxylases (ADXs), three aldehyde reductases (ALRs), ADG Burkholderia sp., AraD Sulfolobus solfataricus, KivD Lactococcus lactis IFPL730, AdhP were bio-production 48 h catalysis, 0.88 g/L produced by recombinant strain BT5. Once pathway, metabolic engineering strategy conducted further improvement. The final BT5ΔyiaEΔycdWΔyagE 1.13 after catalyzing h. Finally, fermentation conditions characteristics also evaluated, then 2.24 obtained catalysis under conditions. Our work first report on which provided potential large-scale production d-glucose-based