Molecular Characteristics of m6A Regulators and Tumor Microenvironment Infiltration in Soft Tissue Sarcoma: A Gene-Based Study

• DOI: 10.3389/fbioe.2022.846812
• Publication Date: 2022-04-19T04:55:17Z
• Authors: Kang-Wen Xiao; Zhi-Qiang Yang; Xin Yan; Zhi-Bo Liu; Min Yang; Liang-Yu Guo; Lin Cai
• Keywords: 0301 basic medicine; 03 medical and health sciences; soft tissue sarcoma; tumor microenvironment; Bioengineering and Biotechnology; m6A; immunotherapy; survival; TP248.13-248.65; Biotechnology; 3. Good health

Background: N6-methyladenosine (m6A) methylation played a key role in tumor growth. However, the relationship between m6A and soft tissue sarcoma (STS) was still unclear. Methods: The characterization patterns of modification STS (TCGA-SARC GSE17674) were analyzed comprehensively through bioinformatics real-time polymerase chain reaction (RT-PCR). effects different on prognosis immune infiltration further explored. Differentially expressed gene (DEG) analysis performed. Moreover, an m6Ascore constructed by principal component (PCA). In addition, two immunotherapy datasets (IMvigor210 GSE78220) dataset (GSE17618) used to evaluate m6Ascore. Results: Huge differences found somatic mutation, CNV, expression 25 regulators STS. Two (A B) identified cluster A showed better clinical outcome with lower immune/stromal score compared B (p < 0.050).In addition , most samples from high m6Ascore, which related mismatch repair 0.001). contrast, B, characterized low MYC signaling pathway, led poor also contributed PD-1/PD-L1 blockade immunotherapy. Conclusion: microenvironment explored comprehensively. novel effectively predicted characteristics (TME) provided insights for future