Interaction of Metallic Nanoparticles With Biomimetic Lipid Liquid Crystalline Cubic Interfaces

• DOI: 10.3389/fbioe.2022.848687
• Publication Date: 2022-03-15T05:24:35Z
• Authors: Jacopo Cardellini; Costanza Montis; Francesco Barbero; Ilaria De Santis; Lucrezia Caselli; Debora Berti
• Keywords: Quartz Crystal Microbalance; Nanomaterials; Nanoclusters

In the past decades, events occurring at nano-bio interface (i.e., where engineered nanoparticles (NPs) meet biological interfaces such as biomembranes) have been intensively investigated, to address cytotoxicity of nanomaterials and boost their clinical translation. this field, lamellar synthetic model membranes instrumental disentangle non-specific interactions between NPs planar interfaces. Much less is known on nano-biointeractions highly curved interfaces, cubic membranes. These non-lamellar architectures play a crucial -but far from understood-role in several processes occur cells defence mechanism against bacterial viral pathologies, including coronaviruses infections. Despite its relevance, interaction with nano-sized objects (such pathogens, macromolecules NPs) remains largely unexplored date. Here, we lipid phase two prototypical classes for Nanomedicine, i.e., gold (AuNPs) silver (AgNPs). To purpose, challenged membranes, either form dispersed cubosomes) or solid-supported layers nanometric thickness, citrate-stabilized AuNPs AgNPs monitored combining bulk techniques (UV-visible spectroscopy, Light Synchrotron Small-Angle X-ray Scattering) surface methods (Quartz Crystal Microbalance Confocal Laser Scanning Microscopy). We show that composition metal core Au vs Ag) modulates adsorption self-assembly leading an extensive membrane-induced clustering AuNPs, while only mild isolated AgNPs. Such differences mirror opposite effects membrane level, induce extraction followed by fast disruption assembly, do not affect morphology. Finally, propose accounting different behaviour interface, highlighting prominent role NPs' chemistry overall mechanism.