Osteogenesis of Iron Oxide Nanoparticles-Labeled Human Precartilaginous Stem Cells in Interpenetrating Network Printable Hydrogel

• DOI: 10.3389/fbioe.2022.872149
• Publication Date: 2022-04-29T06:37:35Z
• Authors: Wei Liao; Jingwei Lu; Qianjin Wang; Sen Yan; Yan Li; Yibo Zhang; Peng Wang; Qing Jiang; Ning Gu
• Keywords: 0301 basic medicine; human precartilaginous stem cells; 03 medical and health sciences; bone regeneration; interpenetrating network hydrogel; iron oxide nanoparticles; osteogenic activity; Bioengineering and Biotechnology; TP248.13-248.65; Biotechnology; 3. Good health

Smart biomaterials combined with stem cell-based therapeutic strategies have brought innovation in the field of bone tissue regeneration. However, little is known about precartilaginous cells (PCSCs), which can be used as seed and incorporated bioactive scaffolds for reconstructive therapy defects. Herein, iron oxide nanoparticles (IONPs) were employed to modulate fate PCSCs, resulting enhanced osteogenic differentiation potential both vitro vivo. PCSCs isolated from ring La-Croix extracted polydactylism patient identified through immunohistochemically staining using anti-FGFR-3 antibodies. Potential toxicity IONPs toward was assessed cell viability, proliferation, attachment assay, results demonstrated that exhibited excellent biocompatibility. After that, effects on evaluated ALP activity, formation mineralized nodule, osteogenic-related genes expressions could observed upon treatment. Moreover, vivo regeneration assessment performed rabbit femur defects a model. A novel methacrylated alginate 4-arm poly (ethylene glycol)-acrylate (4A-PEGAcr)-based interpenetrating polymeric printable network (IPN) hydrogel prepared incorporation IONPs-labeled where 4A-PEGAcr common component three-dimensional (3D) printing. The implantation significantly accelerated process, indicating endow current ability. Together fact PCSCs-incorporated IPN (PCSCs-hydrogels) biosafety printable, we believed PCSCs-hydrogels bioactivity enrich