Lycorine Inhibits Hypertrophic Scar Formation by Inducing ROS-Mediated Apoptosis

• DOI: 10.3389/fbioe.2022.892015
• Publication Date: 2022-05-24T08:06:44Z
• Authors: Yunxian Dong; Dongming Lv; Zirui Zhao; Zhongye Xu; Zhicheng Hu; Bing Tang
• Keywords: Lycorine; Viability assay

Background: Hypertrophic scar (HS) is a fibrotic cutaneous disease with few effective therapies. Lycorine drug pro-apoptotic ability and anti-fibrosis potential. This study aimed to test whether lycorine could trigger the apoptosis of hypertrophic fibroblasts (HSFs) inhibit HS formation. Methods: The proapoptotic effects on viability human primary HSFs their reactive oxygen species (ROS) production as well rabbit ear model were determined by CCK-8, flow cytometry, Western blot, immunofluorescence, transwell migration, collagen gel contraction assays. Results: treatment selectively decreased HSFs, induced apoptosis, but not normal (NFs). increased relative levels Bax cleaved PARP expression, cytochrome C cytoplasm translocation, Bcl-2, caspase-3 caspase-9 mitochondrial membrane potential (MMP) in HSFs. inhibited migration reduced expression I, III α-SMA. Mechanistically, stimulated high ROS production, leading while NAC, inhibitor, significantly mitigated or abrogated antifibrotic activity Moreover, severity ears inducing fibroblast apoptosis. Conclusion: These results indicate that has potent anti-fibrotic for intervention HS.