3D-printed mesoporous bioactive glass/GelMA biomimetic scaffolds for osteogenic/cementogenic differentiation of periodontal ligament cells

• DOI: 10.3389/fbioe.2022.950970
• Publication Date: 2022-10-18T07:05:23Z
• Authors: Nianrou Mei; Yiwen Wu; Binglin Chen; Tian Zhuang; Xinge Yu; Baiyan Sui; Tingting Ding; Xin Liu
• Keywords: Bioactive Glass; Periodontal fiber; Biomaterial; Regenerative Medicine; Gelatin

Integrated regeneration of periodontal tissues remains a challenge in current clinical applications. Due to the tunable physical characteristics and precise control scaffold microarchitecture, three-dimensionally (3D) printed gelatin methacryloyl (GelMA)-based has emerged as promising strategy for tissue regeneration. However, optimization printing biomaterial links formulation relationship between composition structures scaffolds their comprehensive properties (e.g. mechanical strength, degradation, biological behaviors) unclear. Here, this work, novel mesoporous bioactive glass (BG)/GelMA biomimetic with large pore size (∼300 μm) was developed by extrusion-based 3D printing. Our results showed that incorporation nanoparticles (BG NPs) significantly improved shape fidelity, surface roughness, bioactivity 3D-printed macroporous GelMA scaffolds, resulting enhanced effects on cell attachment promoting osteogenic/cementogenic differentiation human ligament cells. The excellent maintenance macropore structure, visibly cells spreading, release ions (Si4+, Ca2+), upregulation gene expressions osteogenesis cementogensis, increase alkaline phosphatase (ALP) activity calcium nodules suggested BG NPs could endow GelMA-based structural stability ability promote differentiation. findings demonstrated great potential newly formulated inks BG/GelMA being used provide important insights into understanding cell-scaffold interaction functional tissues.