Crowding-induced morphological changes in synthetic lipid vesicles determined using smFRET

• DOI: 10.3389/fbioe.2022.958026
• Publication Date: 2022-10-28T06:13:34Z
• Authors: Steven D. Quinn; Lara Dresser; Sarah Graham; Donato Conteduca; Jack Shepherd; Mark C. Leake
• Keywords: Lipid vesicle; Crowding

Lipid vesicles are valuable mesoscale molecular confinement vessels for studying membrane mechanics and lipid-protein interactions, they have found utility among bio-inspired technologies, including drug delivery vehicles. While vesicle morphology can be modified by changing the lipid composition introducing fusion or pore-forming proteins detergents, influence of extramembrane crowding on has remained under-explored owing to a lack experimental tools capable capturing morphological changes nanoscale. Here, we use biocompatible polymers simulate