Enhanced production of iturin A by strengthening fatty acid synthesis modules in Bacillus amyloliquefaciens

• DOI: 10.3389/fbioe.2022.974460
• Publication Date: 2022-09-09T06:59:32Z
• Authors: Lin Gao; Menglin She; Jiao Shi; Dongbo Cai; Dong Wang; Min Xiong; Guoming Shen; Jiaming Gao; Min Zhang; Zhifan Yang; Shouwen Chen
• Keywords: Bacillus amyloliquefaciens; Strain (injury); Fatty acid synthesis; Corynebacterium glutamicum; Bacillus (shape)

Iturin A is a biosurfactant with various applications, and its low synthesis capability limits production application development. Fatty acids play critical role in cellular metabolism target product syntheses, the relationship between fatty acid supplies iturin unclear. In this study, we attempted to increase via strengthening pathways Bacillus amyloliquefaciens. First, acetyl-CoA carboxylase AccAD ACP S-malonyltransferase fabD were overexpressed promoter replacement, yield was increased 1.36 g/L by 2.78-fold resultant strain HZ-ADF1. Then, soluble acyl-ACP thioesterase derived from Escherichia coli showed best performance for synthesis, as compared those B. amyloliquefaciens Corynebacterium glutamicum, introduction of which HZ-ADF1 further led 57.35% yield, reaching 2.14 g/L. Finally, long-chain acid-CoA ligase LcfA HZ-ADFT attain final HZ-ADFTL2, reached 2.96 g/L, increasing 6.59-fold, contents enhanced significantly original HZ-12. Taken together, our results implied that an efficient approach production, research provided promising industrial A.