Biological Evaluation of Acellular Cartilaginous and Dermal Matrixes as Tissue Engineering Scaffolds for Cartilage Regeneration

• DOI: 10.3389/fcell.2020.624337
• Publication Date: 2021-01-12T23:50:33Z
• Authors: Yahui Wang; Yong Xu; Guangdong Zhou; Yu Liu; Yilin Cao
• Keywords: Autologous chondrocyte implantation; Biomaterial; Matrix (chemical analysis); Aggrecan

An acellular matrix (AM) as a kind of natural biomaterial is gaining increasing attention in tissue engineering applications. cartilaginous (ACM) and dermal (ADM) are two kinds the most widely used AMs cartilage engineering. However, there still debate over which these achieves optimal regeneration, especially immunocompetent large animals. In current study, we fabricated porous ADM ACM scaffolds by freeze-drying method confirmed that had larger pore size than ACM. By recolonization with goat auricular chondrocytes vitro culture, exhibited higher cell adhesion rate, more homogeneous chondrocyte distribution, neocartilage formation compared Additionally, quantitative polymerase chain reaction (qPCR) indicated expression cartilage-related genes, including ACAN, COLIIA1, SOX9, was significantly group group. Furthermore, after subcutaneous implantation goat, histological evaluation showed achieved stable matured ACM, data wet weight, volume, contents DNA, GAG, total collagen, collagen II. immunological assessment suggested evoked low immune response evidenced qPCR immunohistochemical analyses CD3 CD68, TUNEL. Collectively, our results indicate suitable AM for can be regeneration