Adipose-Derived Stromal Cell-Sheets Sandwiched, Book-Shaped Acellular Dermal Matrix Capable of Sustained Release of Basic Fibroblast Growth Factor Promote Diabetic Wound Healing

• DOI: 10.3389/fcell.2021.646967
• Publication Date: 2021-03-25T08:22:04Z
• Authors: Xin Shi; Liyuan Jiang; Xin Zhao; Bei Chen; Wei Shi; Yanpeng Cao; Yaowu Chen; Xiying Li; Yusheng He; Chengjie Li; Xiaoren Liu; Xing Li; Hongbin Lu; Can Chen; Jun Liu
• Keywords: Granulation tissue; Decellularization

The management of diabetic wounds is a therapeutic challenge in clinical settings. Current tissue engineering strategies for wound healing are insufficient, owing to the lack an appropriate scaffold that can load large number stem cells and induce interaction form granulation tissue. Herein we fabricated book-shaped decellularized dermal matrix (BDDM), which shows high resemblance native terms its histology, microstructure, ingredients, non-cytotoxic low-immunogenic, allows adipose-derived stromal cell (ASC) attachment proliferation. Then, collagen-binding domain (CBD) capable binding collagen was fused into basic fibroblast growth factor (bFGF) synthetize recombinant (termed as CBD–bFGF). After that, CBD–bFGF tethered onto fibers BDDM improve endothelial inducibility. Finally, functional (CBD–bFGF/BDDM) fabricated. In vitro vivo experiments demonstrated CBD–bFGF/BDDM release bFGF with sustained profile, steadily inducing down differentiation. ASCs were cultured sheet then sandwiched by CBD–bFGF/BDDM, thus enlarging loaded scaffold. Using rat model, ASC sheets (ASCs/CBD–bFGF/BDDM) enhancing formation tissue, promoting angiogenesis, facilitating deposition remodeling. Therefore, findings this study demonstrate ASCs/CBD–bFGF/BDDM could be applicable healing.