Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa

• DOI: 10.3389/fcell.2021.652809
• Publication Date: 2021-07-16T11:47:18Z
• Authors: Yongjie Xu; Qiu Han; Chaofeng Ma; Yaling Wang; Pengpeng Zhang; Cencen Li; Xiaofang Cheng; Haixia Xu
• Keywords: Capacitation; Phosphoproteomics; BOAR; Proteome; Gamete

Sperm cells are of unique elongated structure and function, the development which is tightly regulated by existing proteins posttranslational modifications (PTM) these proteins. Based on phylogenetic relationships various swine breeds, Yorkshire boar believed to be distinctly different from Duroc boar. The comprehensive differential proteomics phosphoproteomics profilings were performed spermatozoa both boars. By peptide PTM quantification followed statistical analyses, 167 differentially expressed identified 1,745 proteins, 283 phosphopeptides corresponding 102 phosphorylated measured 1,140 derived 363 representative results validated Western blots. Pathway enrichment analyses revealed that majority expression phosphorylation primarily concerned with spermatogenesis, male gamete generation, sperm motility, energy metabolism, cilium morphogenesis, axonemal dynein complex assembly, sperm–egg recognition, capacitation. Remarkably, assembly related such as SMCP, SUN5, ODF1, AKAP3, AKAP4 play a key regulatory role in physiological functions, significantly higher than Yorkshire. Furthermore, sperm-specific CABYR, ROPN1, CALM1, PRKAR2A, PRKAR1A, participates regulation motility mainly through cAMP/PKA signal pathway demonstrating protein may an important mechanism underlying diversity. Protein–protein interaction analysis 14 overlapped between potentially played flagellum, including AKAP4, FSIP2, SUN5. Taken together, physiologically functionally (DEPs) (DPPs) constitute proteomic backgrounds two breeds. validation will delineate roles modulators spermatozoa.