BRCC3 Promotes Tumorigenesis of Bladder Cancer by Activating the NF-κB Signaling Pathway Through Targeting TRAF2

• DOI: 10.3389/fcell.2021.720349
• Publication Date: 2021-09-16T04:45:29Z
• Authors: Huangheng Tao; Yixiang Liao; Youji Yan; Zhiwen He; Jiajie Zhou; Xinghuan Wang; Jianping Peng; Shangze Li; Tao Liu
• Keywords: TRAF2

NF-κB signaling is very important in cancers. However, the role of BRCC3-associated activation bladder cancer remains to be characterized. Western blotting and IHC tissue microarray were used confirm abnormal expression BRCC3 cancer. Growth curve, colony formation, soft agar assay Xenograft model performed identify over-expression or knock-out Further, RNA-Seq luciferase reporter assays down-stream pathway. Finally, co-immunoprecipitation fluorescence confocal verify precise target BRCC3. Here, we found that high promoted tumorigenesis through targeting TRAF2 protein. up-regulated patients which indicates a negative prognosis. By vitro vivo assays, genetic ablation markedly blocks proliferation, viability migration cells. Mechanistically, analysis shows down-regulated BRCC3-deficient binds synergizes with activate signaling. Our results indicate activates by for activation, turn facilitates This finding points as potential patients.