Senescent Tissue-Resident Mesenchymal Stromal Cells Are an Internal Source of Inflammation in Human Osteoarthritic Cartilage

• DOI: 10.3389/fcell.2021.725071
• Publication Date: 2021-09-06T19:17:13Z
• Authors: Wenguang Liu; Alexander S. Brodsky; Meng Feng; Yajun Liu; Jing Ding; Chathuraka T. Jay...; Qian Chen
• Keywords: Senescence; Chondrogenesis

Human osteoarthritic cartilage contains not only chondrocytes (OACs), but also mesenchymal stromal cells (OA-MSCs), whose abundance increases during osteoarthritis (OA). However, it is clear how OA-MSC contributes to OA pathogenesis. Here, we show that aging plays an important role in cell senescence, fibrosis, and inflammation cartilage. Protein array analysis indicates expresses pro-inflammatory senescence associated secretory phenotype (SASP) including IL-1β, IL-6, IL-8, CXCL1, 5, 6, which play key roles OAC a main recipient of the inflammatory signals by expressing receptors cytokines. RNAseq transition from normal (NCSCs) results activation SASP gene expression. This process can be recapitulated serial passage primary culture comprising (1) dedifferentiation into NCSC-like cells, (2) its subsequent OA-MSC. While mediated transcriptional repression chondrogenic expression, We postulate that, through replication-driven (MSC) becomes internal source sterile human joint.