Transcriptome Dynamics of Human Neuronal Differentiation From iPSC

• DOI: 10.3389/fcell.2021.727747
• Publication Date: 2021-12-14T10:58:13Z
• Authors: Meltem Kuruş; Soheil Akbari; Doğa Eskier; Ahmet Bursalı; Kemal Ergin; Esra Erdal; Gökhan Karakülah
• Keywords: Gene regulatory network

The generation and use of induced pluripotent stem cells (iPSCs) in order to obtain all differentiated adult cell morphologies without requiring embryonic is one the most important discoveries molecular biology. Among uses iPSCs neuron organoids study biological cues underlying neuronal brain development, addition neurological diseases. These iPSC-derived differentiation models allow us examine gene regulatory factors involved such processes. these are long non-coding RNAs (lncRNAs), genes that transcribed from genome have key functions establishing phenotypes, but frequently not included studies focusing on protein coding genes. Here, we provide a comprehensive analysis overview transcriptome during multiple stages process using RNA-seq. We identify previously unannotated lncRNAs via genome-guided de novo assembly, distinct characteristics each stage, including differentially expressed stage specific further human network, representing novel candidates likely critical roles neurogenesis coexpression network analysis. Our findings valuable resource for future differentiation.