Microbiomes and Resistomes in Biopsy Tissue and Intestinal Lavage Fluid of Colorectal Cancer

• DOI: 10.3389/fcell.2021.736994
• Publication Date: 2021-09-17T05:15:08Z
• Authors: Yumeng Yuan; Yihuan Chen; Fen Yao; Mi Zeng; Qingdong Xie; Muhammad Shafiq; Sohail Muhammad N...; Xiaoyang Jiao
• Keywords: Prevotella

Aim: The gut microbiome plays a crucial role in colorectal cancer (CRC) tumorigenesis, but compositions of microorganisms have been inconsistent previous studies due to the different types specimens. We investigated microbiomes and resistomes CRC patients with colonic biopsy tissue intestinal lavage fluid (IVF). Methods: Paired samples (biopsy IVF) were collected from 20 CRC, their measured by shotgun metagenomics. Clinical laboratory data recorded. Bioinformatics (KneadData, Kraken2, FMAP) statistical analysis done using R (v4.0.2) software. Results: Bacterial diversity IVF was higher than samples, bacterial operational taxonomic units (OTUs) 2,757 vs. 197 tissue. β-diversity showed distinct clusters paired samples. predominant bacteria phylum Proteobacteria, while Actinobacteria. Twenty-seven representative selected form six clusters, which only Firmicutes Cluster 1, Bacteroidetes 1 significantly more abundant group those ( p < 0.05). 2, Pathogen Cluster, Prevotella not between > Correlation revealed that some could effects on metabolic inflammatory parameters patients. A total 1,295 antibiotic resistance genes (ARGs) detected microbiomes, conferred multidrug resistance, as well tetracycline, aminoglycoside, more. Co-occurrence patterns network mainly ARG-carrying be similar tissue, leading located hub differed Conclusion: Heterogeneity microbiota is particularly evident when studied close relationships carcinogenesis are different, an alternative for microbiome, resistome assessment may feasible method.