Validation of Effective Extracellular Vesicles Isolation Methods Adapted to Field Studies in Malaria Endemic Regions

• DOI: 10.3389/fcell.2022.812244
• Publication Date: 2022-05-16T06:17:06Z
• Authors: Matteo Zoia; Bibin Yesodha Sub...; Klara Kristin Eri...; Meera Sruthi Ravi; Shekoofeh Yaghmaei; Isabelle Fellay; Brigitte Scolari; Michael Walch; Pierre-Yves Mantel
• Keywords: Extracellular Vesicles

Malaria affects the poorer regions of world and is tremendous health economic burden for developing countries. Extracellular vesicles (EVs) are small released by almost any cells in human body, including malaria infected red blood cells. Recent evidence shows that EVs might contribute to pathogenesis malaria. In addition, hold considerable value biomarker discovery. However, there still significant gaps our understanding EV biology. So far most knowledge about comes from vitro work. More field studies required gain insight into their contribution disease under physiological conditions. perform research on low-income be challenging due lack appropriate equipment isolate EVs. Therefore, a need develop validate extraction protocols applicable poorly equipped laboratories. We established validated two isolation cell culture supernatants, rodent plasma. compared polyethylene glycol (PEG) salting out (SA) with sodium acetate precipitation then characterized Transmission Electron Microscopy (TEM), Western Blot, Size-exclusion chromatography (SEC), bead-based flow cytometry protein quantification. Both resulted efficient purification without expensive material or ultracentrifugation. Furthermore, procedure easily scalable work large sample volumes. Here, we propose both approaches can used resource limited countries, therefore further helping close gap during