Brevican, Neurocan, Tenascin-C, and Tenascin-R Act as Important Regulators of the Interplay Between Perineuronal Nets, Synaptic Integrity, Inhibitory Interneurons, and Otx2

• DOI: 10.3389/fcell.2022.886527
• Publication Date: 2022-06-02T11:28:31Z
• Authors: Cornelius Mueller...; Jacqueline Reinhard; Lars Roll; Verian Bader; Konstanze F. Wink...; Andreas Faissner
• Keywords: Perineuronal net; Interneuron; Calretinin

Fast-spiking parvalbumin interneurons are critical for the function of mature cortical inhibitory circuits. Most these neurons enwrapped by a specialized extracellular matrix (ECM) structure called perineuronal net (PNN), which can regulate their synaptic input. In this study, we investigated relationship between PNNs, interneurons, and distribution on cells in adult primary visual cortex (V1) quadruple knockout mice deficient ECM molecules brevican, neurocan, tenascin-C, tenascin-R. We used super-resolution structured illumination microscopy (SIM) to analyze PNN associated synapses. addition, examined calretinin interneuron populations. observed reduction number PNN-enwrapped clear disorganization V1. This was accompanied an imbalance excitatory synapses with increase elements along PNNs. Furthermore, reduced knockout, while do not wear did display differences number. Interestingly, found transcription factor Otx2 homeoprotein positive cell population also reduced. is crucial maturation, feedback loop parameters has been described. Collectively, data indicate important role tenascin-R regulating interplay distribution,