CD34+ HSPCs-derived exosomes contain dynamic cargo and promote their migration through functional binding with the homing receptor E-selectin

• DOI: 10.3389/fcell.2023.1149912
• Publication Date: 2023-04-25T04:28:31Z
• Authors: Ioannis Isaioglou; Mansour M. Aldeha...; Yanyan Li; Abdellatif Ait La...; Sakandar Rauf; Asma S. Al-Amoodi; Umme Habiba; Abdullah Alghamdi; Shuho Nozue; Satoshi Habuchi; Khaled N. Salama; Jasmeen S. Merzaban
• Keywords: Homing (biology); Exosome

Exosomes are tiny vesicles released by cells that carry communications to local and distant locations. Emerging research has revealed the role played integrins found on surface of exosomes in delivering information once they reach their destination. But until now, little been known initial upstream steps migration process. Using biochemical imaging approaches, we show here isolated from both leukemic healthy hematopoietic stem/progenitor can navigate way cell origin due presence sialyl Lewis X modifications glycoproteins. This, turn, allows binding E-selectin at sites so deliver messages. We when were injected into NSG mice, traveled spleen spine, typical engraftment. This process, however, was inhibited mice pre-treated with blocking antibodies. Significantly, our proteomic analysis among proteins contained within signaling proteins, suggesting trying active cues recipient potentially alter physiology. Intriguingly, work outlined also suggests protein cargo dynamically change upon exosome receptors such as E-selectin, which thereby could impact it regulate physiology cells. Furthermore, an example how miRNAs influence RNA expression cells, showed KG1a-derived target tumor suppressing PTEN.